StrepII Tag Mouse mAb (HRP Conjugated)(StrepII Tag 小鼠单抗)(AF2927)

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StrepII Tag Mouse mAb (HRP Conjugated)(StrepII Tag 小鼠单抗)(AF2927)

2024-02-22 06:29| 来源: 网络整理| 查看: 265

来源用途交叉反应性理论分子量实际分子量 Mouse WB, IHC, ICC StrepII-tagged protein in all species - -

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB IP IF IHC ICC FC ChIP ChIP-seq 1:500-1:2000 - - 1:50-1:100 1:50-1:100 - - -

抗体详细信息如下:

About this Antibody Name StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) Category Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody Isotype IgG1 Purificationmethod Affinity purification Positive samples - Cellular location - Customer validation - About the Immunogen Immunogen Synthetic StrepII tag peptide coupled to KLH Sequence WSHPQFEK Gene ID - SwissProt - Synonyms - Category Polypeptide protein tag Background Streptavidin is a tetrameric protein expressed in Streptomyces avidinii. Because of its high affinity for the vitamin h-biotin, Streptavidin is commonly used in the fields of molecular biology and biotechnology. The Strep-tag was originally selected from a genetic library to specifically bind to a proteolytically truncated "core" version of streptavidin. Over the years, the Strep -tag was systemically optimized, to permit a greater flexibility in the choice of attachment site. Further, its interaction partner, Streptavidin was also optimized to increase peptide-binding capacity, which resulted in the development of Strep-Tactin. The so called Strep-tag system, consisting of Strep-tag and Strep-Tactin, has proven particularly useful for the functional isolation and analysis of protein complexes in proteome research. 包装清单：产品编号产品名称包装 AF2927 StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) 50µl AZ050 Western一抗稀释液 50ml — 说明书 1份保存条件：

StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项：

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。

回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

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StrepII Tag Mouse mAb (HRP Conjugated)(StrepII Tag 小鼠单抗)(AF2927)

StrepII Tag Mouse mAb (HRP Conjugated)(StrepII Tag 小鼠单抗)(AF2927)

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